rat primary dermal fibroblasts rdfs  (Procell Inc)

Journal: International Dental Journal

Article Title: Anti-Radiofibrosis Effect of Dicliptera chinensis Polysaccharide on Rat Dermal Fibroblasts Via The TGF-β1/Smads/CTGF Signaling Pathway

doi: 10.1016/j.identj.2024.09.024

Figure Lengend Snippet: Effects of different Dicliptera chinensis polysaccharide (DCP) concentrations on the viability of rat dermal fibroblasts (RDFs). CCK8 assay was used to examine the role of DCP in RDF cell lines. Cells were inoculated into 96-well plates and exposed to the specified concentrations of DCP (50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL, 800 μg/mL, and 1.6 mg/mL) for 24 hours. The result shown is the standard deviation average of 3 independent experiments. The significance was analyzed using one-way analysis of variance with Dunnett-t test to compare the means between the control and experimental groups (* P < .05, ⁎⁎ P < .01, ⁎⁎⁎ P < .001 and blank group).

Article Snippet: Primary rat dermal fibroblasts (RDFs) were purchased from Procell Corp.

Techniques: CCK-8 Assay, Standard Deviation, Control

Journal: International Dental Journal

Article Title: Anti-Radiofibrosis Effect of Dicliptera chinensis Polysaccharide on Rat Dermal Fibroblasts Via The TGF-β1/Smads/CTGF Signaling Pathway

doi: 10.1016/j.identj.2024.09.024

Figure Lengend Snippet: Effect of Dicliptera chinensis polysaccharide (DCP) on radiation-induced apoptosis of rat dermal fibroblasts (RDFs). (A) RDFs were cultured in 100 μg/mL and 200 μg/mL DCP with experimental concentrations for 24 hours and then irradiated with 8 GY X-rays. The cells were then stained with Annexin V- Fluorescein Isothiocyanate and propium iodide and detected by flow cytometry. (B) The result shown in B is the standard deviation average of 3 independent experiments. The significance was determined using one-way analysis of variance (* P < .05, ⁎⁎ P < .01, ⁎⁎⁎ P < .001 and ionising radiation [IR] group).

Article Snippet: Primary rat dermal fibroblasts (RDFs) were purchased from Procell Corp.

Techniques: Cell Culture, Irradiation, Staining, Flow Cytometry, Standard Deviation

Journal: International dental journal

Article Title: Anti-Radiofibrosis Effect of Dicliptera chinensis Polysaccharide on Rat Dermal Fibroblasts Via The TGF-β1/Smads/CTGF Signaling Pathway.

doi: 10.1016/j.identj.2024.09.024

Figure Lengend Snippet: Figure 1 – Effects of different Dicliptera chinensis polysaccharide (DCP) concentrations on the viability of rat dermal fibro- blasts (RDFs). CCK8 assay was used to examine the role of DCP in RDF cell lines. Cells were inoculated into 96-well plates and exposed to the specified concentrations of DCP (50 mg/mL, 100 mg/mL, 200 mg/mL, 400 mg/mL, 800 mg/mL, and 1.6 mg/mL) for 24 hours. The result shown is the standard deviation average of 3 independent experiments. The significance was analyzed using one-way analysis of variance with Dunnett-t test to compare the means between the control and experimental groups (*P < .05, **P < .01, *** P < .001 and blank group).

Article Snippet: Primary rat dermal fibroblasts (RDFs) were purchased from Procell Corp.

Techniques: CCK-8 Assay, Standard Deviation, Control

Journal: International dental journal

Article Title: Anti-Radiofibrosis Effect of Dicliptera chinensis Polysaccharide on Rat Dermal Fibroblasts Via The TGF-β1/Smads/CTGF Signaling Pathway.

doi: 10.1016/j.identj.2024.09.024

Figure Lengend Snippet: Figure 2 – Effect of Dicliptera chinensis polysaccharide (DCP) on radiation-induced apoptosis of rat dermal fibroblasts (RDFs). (A) RDFs were cultured in 100 mg/mL and 200 mg/mL DCP with experimental concentrations for 24 hours and then irradiated with 8 GY X-rays. The cells were then stained with Annexin V- Fluorescein Isothiocyanate and propium iodide and detected by flow cytometry. (B) The result shown in B is the standard deviation average of 3 independent experiments. The signifi- cance was determined using one-way analysis of variance (*P < .05, **P < .01, *** P < .001 and ionising radiation [IR] group).

Article Snippet: Primary rat dermal fibroblasts (RDFs) were purchased from Procell Corp.

Techniques: Cell Culture, Irradiation, Staining, Cytometry, Standard Deviation

Journal: Cell reports

Article Title: Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells

doi: 10.1016/j.celrep.2020.107711

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cryopreserved rat dermal fibroblast , Cell Applications, Inc , R106-05a.

Techniques: Recombinant, Concentration Assay, Knock-Out, Membrane, SYBR Green Assay, Acid Assay, MTT Assay, DNA Purification, Enzyme-linked Immunosorbent Assay, Hydroxyproline Assay, Colorimetric Assay, Binding Assay, Software, Micro-CT, Multiplex sample analysis, Transmission Assay, Microscopy, Micro-MRI, High Performance Liquid Chromatography